Coding

Part:BBa_K2984035:Design

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-Psad-PETase-YFP-His-RbcS2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1244
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
    Illegal NgoMIV site found at 2396
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PETase Plasmid designed for Transformation in Clamydomnasd and further cloning into Level 2 backbone with MoClo Standard.



Source

The construct consist of the four different Level 0 parts Psad (BBa_K2984008),PETase(), YFP (BBa_K2984017), His-Tag ( BBa_K2984014) Rbcs2 (BBa_K2984018) which were cloned into the level 1 Backbone (BBa_K2984002) using the MoClo Standard.


References